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PrimeTime™ Pathogen Research Panels
Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.
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PrimeTime™ Pathogen Research Panels
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PrimeTime™ Pathogen Research Panels
Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.
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ReadyMade Oligos
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PrimeTime™ Pathogen Research Panels
Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.
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qPCR
PrimeTime™ Pathogen Research Panels
Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.
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qPCR
PrimeTime™ Sexual Health Research Panels
Optimized sexual health panels remove sequence design guesswork—saving time and money with flexible fluorophore options, platform compatibility, and reliable results.
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Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.
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HDR Reagents
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rhAmpSeq™ CRISPR Data Analysis System
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Genome Editing Detection Kit
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Accelerate your CRISPR therapeutics with our full CGMP guide RNA manufacturing/regulatory services.
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CRISPR
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Gene Synthesis & Fragments
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gBlocks HiFi Gene Fragments
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Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.
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Gene Regulation
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Increase stability and reduce toxicity with nuclease-resistant mods and flexible chimeric designs.
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Reagents
DsiRNAs for RNAi
Effectively target cytoplasmic mRNA and noncoding RNA with predesigned or custom RNAi solutions.
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GMP Oligo Manufacturing
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Accelerate your CRISPR therapeutics with our full CGMP guide RNA manufacturing/regulatory services.
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Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.
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Fundamental concepts, innovations, and insights about CRISPR R&D and the therapeutic landscape.
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gBlocks HiFi Gene Fragments
Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.
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gBlocks HiFi Gene Fragments
Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.
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gBlocks HiFi Gene Fragments
Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.
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UNAFold Tool
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CRISPR Genome Editing
Alt-R Predesigned Cas9 Selection Tool
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CRISPR-Cas9 Design Checker
Alt-R CRISPR HDR Design Tool
rhAmpSeq Design Tool
rhAmpSeq™ CRISPR Data Analysis Tool
CRISPR
CRISPR Cas12a
Open up CRISPR editing to additional areas in genomes.
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CRISPR
CRISPR-Cas9 Systems
Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.
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qPCR Assay Design
PrimerQuest™ Tool
Realtime PCR Tool
PrimeTime™ Predesigned Gene Expression Assays
PrimeTime Multiplex Dye Selection
qPCR
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Multiplex your way with a wide range of high-efficiency quenchers, dyes, and scales.
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qPCR
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Gene Regulation and RNAi
Predesigned DsiRNA Selection Tool
RNAi Design Tool
Oligos
Antisense Oligonucleotides
Increase stability and reduce toxicity with nuclease-resistant mods and flexible chimeric designs.
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Reagents
DsiRNAs for RNAi
Effectively target cytoplasmic mRNA and noncoding RNA with predesigned or custom RNAi solutions.
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Genotyping
rhAmp Genotyping Design Tool
qPCR
Custom qPCR Probes
Multiplex your way with a wide range of high-efficiency quenchers, dyes, and scales.
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Genes & Gene Fragments
Codon Optimization Tool
gBlocks Gene Fragments Entry Tool
Genes & Gene Fragments order status
Genes
Rapid Genes
Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.
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SciTools™ Web Tools: Your gateway to discovery

Streamline oligonucleotide design and experimental planning with powerful, user-friendly tools and calculators, trusted by researchers worldwide.

Oligo design & handling

Streamline oligonucleotide design and experimental planning with powerful, user-friendly tools and calculators, trusted by researchers worldwide.

OligoAnalyzer™ Tool
UNAFold Tool
Resuspension Calculator
Dilution Calculator
PrimerQuest™ Tool
FEATURED RESOURCE

How to use the IDT OligoAnalyzer™ Tool

Analyze and validate oligo designs using step-by-step instructions to generate highly functional and reliable sequences. FEATURED RESOURCE

Tips for resuspending and diluting your oligonucleotides

Accurately resuspend and dilute oligos for consistent and reliable results. FEATURED RESOURCE

Annealing temperature FAQs

Determine, optimize, and set ideal annealing temperatures to prevent off-target binding and improve experimental results.
Jumpstart your next CRISPR genome editing project with expert sequence design and data analysis tools for precise, efficient editing.

Crispr genome editing

Jumpstart your next CRISPR genome editing project with expert sequence design and data analysis tools for precise, efficient editing.

Alt-R™ Predesigned Cas9 guide RNA Selection Tool
Alt-R Custom Cas9 guide RNA Design Tool
CRISPR-Cas9 guide RNA Design Checker
Alt-R CRISPR HDR Design Tool
rhAmpSeq™ Design Tool
rhAmpSeq CRISPR Data Analysis Tool
FEATURED RESOURCE

rhAmpSeq™ CRISPR Data Analysis Tool tutorial

Access CRISPR design proficiency with step-by-step video guidance to analyze and select accurate guide RNA sequences. FEATURED RESOURCE

How to design sgRNA sequences

Design on-target guide RNA sequences for high-efficiency CRISPR editing using simple, expert-backed instructions. FEATURED SUPPORT

Request a CRISPR consult

Get expert support to advance your CRISPR genome editing projects with a free consultation.
Design high-performance qPCR assays with intuitive tools for primer selection, probe customization, and multiplex setup.

qPCR assay design

Design high-performance qPCR assays with intuitive tools for primer selection, probe customization, and multiplex setup.

PrimerQuest™ Tool
Realtime PCR Tool
PrimeTime™ Predesigned Gene Expression Assays
PrimeTime™ Multiplex Dye Selection
FEATURED RESOURCE

How to design primers and probes for PCR and qPCR

Design effective primers and probes using expert guidance and online tools to ensure reliable amplification. FEATURED RESOURCE

Primer design tools for PCR & qPCR

Build PCR and qPCR assays with expert-curated sequences or customize primers and probes for consistent, high-performance results. FEATURED RESOURCE

Real-Time PCR handbook

Learn to design, analyze, and troubleshoot singleplex or multiplex qPCR assays with step-by-step guidance to achieve high-quality results.
Achieve your gene expression modulation goals with simple-to-use design tools, built to minimize off-target risks.

Gene regulation & RNAi

Achieve your gene expression modulation goals with simple-to-use design tools, built to minimize off-target risks.

Predesigned DsiRNA Selection Tool
RNAi Design Tool
FEATURED PRODUCT

DsiRNAs and TriFECTa® Kits

Get predesigned or custom RNAi products, including 27mer DsiRNAs, designed for superior RNA interference compared to 21mer siRNAs.
Optimize your Single Nucleotide Polymorphism (SNP) genotyping workflows with our assay finder and sequence design tools for accurate, high-confidence results.

Genotyping

Optimize your Single Nucleotide Polymorphism (SNP) genotyping workflows with our assay finder and sequence design tools for accurate, high-confidence results.

rhAmp™ Genotyping Design Tool
FEATURED RESOURCE

Genotyping: Terms to know

Master key genetic concepts from genes and alleles to SNPs and mutations with expert-curated explanations. FEATURED PRODUCT

rhAmp SNP Genotyping System

Identify single nucleotide polymorphisms (SNPs) with an all-in-one system designed to deliver high-quality genotyping data.
Transform cloning, gene expression, and synthetic biology workflows with web-based codon analysis software and convenient ordering tools.

Genes & gene fragments

Transform cloning, gene expression, and synthetic biology workflows with web-based codon analysis software and convenient ordering tools.

Codon Optimization Tool
gBlocks™ Gene Fragments Entry Tool
Genes & Gene Fragments order status
FEATURED RESOURCE

IDT Codon Optimization Tool tutorial

Acquire codon optimization expertise with step-by-step video guidance to customize sequences for optimal expression across host systems. FEATURED RESOURCE

(Almost) everything you need to know about gene fragments

Demystify gene fragments with clear, simplified, practical insights to strengthen understanding and guide experimental design. FEATURED PRODUCT

Rapid Genes

Accelerate high-throughput workflows with NGS-verified double-stranded clonal genes.
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Disclaimer

Please use the following format when citing an IDT SciTools Web Tool:

[Tool name] (e.g., PrimerQuest™), IDT, Coralville, Iowa, USA. Accessed 12 May 2025. https://www.idtdna.com/SciTools.