Resuspension Calculator

The Resuspension Calculator assists in determining the volume needed to resuspend a dry, lyophilized oligo to a desired concentration. Simply enter the quantity of oligonucleotide and final desired concentration and click “Calculate.” If you choose a pre-determined Extinction Coefficient or if the Molecular Weight is not required for your calculation, “Not Needed” will be shown.

Results

Quantity

OD260 nmol mg

Final Concentration

µM (µmol/L or pmol/µL) ng/µL (µg/mL or mg/L) µg/µL (mg/mL or g/L) OD260/µL OD260/mL

Preset Extinction Coefficients

Manual Entry Sgl-Stranded DNA, 33µg/OD260 Sgl-Stranded RNA, 40µg/OD260 Dbl-Stranded DNA, 50µg/OD260

Extinction Coefficient Where do I find my EC?

L/(mol·cm)

Molecular Weight