| Specification | Standard desalt purification | HPLC purification |
|---|---|---|
| Length | 30-175nt | |
| Yields |
2, 10, 50, 100 nmol |
|
| Format |
Tubes or plates |
|
| Available modifications & base types | RNA, 2'O-Methyl, phosphorothioate bonds, 2' Fluoro bases, DNA bases, Affinity Plus (locked nucleic acid) bases | |
| QC |
ESI and OD |
ESI-MS, OD and optimized UHPLC purity assessment |
| Shipping times* |
Starting at 3 business days |
Starting at 10 business days |
* Shipping times are for most standard requests. Custom requests may require additional manufacturing time.
| Situation | Guide RNA Suggestions |
|---|---|
| Limited budget, no other constraints | Two-part guide RNA |
| High endo- or exo-nuclease activity in cells | Single guide RNA (first choice) |
| OR (next choice if the first choice does not work), two-part guide RNA with many chemical modifications,* | |
| OR (third choice, if the above choices do not work), single guide RNA with many chemical modifications** | |
| Delivery of pre-formed Cas9 protein | Two-part guide RNA, OR single guide RNA (both choices are equally likely to work) |
| Delivery of mRNA or plasmid coding for Cas9 | Single guide RNA for longer stability |
| Low editing efficiency with single guide RNA | Two-part guide RNA (first choice), |
| OR try another target site (second choice if the first choice does not work) | |
| Low editing efficiency with two-part guide RNA | Single guide RNA (first choice), |
| OR try another target site (second choice if the first choice does not work) |
