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Oligos & Reagents
Custom DNA Oligos
DNA Oligos
Ultramer DNA Oligos
Oligo pools
SameDay Oligos
HotPlates Oligo Service
Rapid HPLC Oligos
TruGrade DNA Oligos
Mixed bases
Affinity Plus DNA Oligonucleotides
Normalized Oligos
Oligos
Custom DNA Oligos
>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.
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Custom RNA Oligos
RNA Oligos
Ultramer RNA Oligos
Affinity Plus RNA Oligonucleotides
Oligos
Custom DNA Oligos
>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.
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Oligo Modifications
Attachment chemistry and linkers
Fluorophores and dark quenchers
Modified bases
Phosphorylation
Spacers
Click Chemistry Modifications
Phosphorothioate bonds
Oligos
Custom DNA Oligos
>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.
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Custom GMP Oligos
Reagents
Nuclease Free Reagents
Nuclease Detection & Control
Oligos
Custom DNA Oligos
>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.
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OligoCard
Oligos
Custom DNA Oligos
>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.
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PCR / qPCR / dPCR
Custom qPCR & dPCR Probes
PrimeTime™ qPCR Probes
PrimeTime™ qPCR Custom Probe Based Assays
Affinity Plus™ qPCR Probes
PrimeTime™ LNA® qPCR Probes
Molecular Beacons
GMP Primers & Probes
MGB Eclipse® Probes
qPCR
PrimeTime™ Pathogen Research Panels
Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.
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Predesigned PCR-based Assays
PrimeTime™ Gene Expression Assays
PrimeTime™ Pathogen Research Panels
PrimeTime™ Sexual Health Research Panels
rhAmp™ SNP Genotyping System
PACE™ SNP Genotyping Assays
Predesigned Gene Expression Assay Tool
qPCR
PrimeTime™ Pathogen Research Panels
Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.
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Master Mixes & Reagents
PrimeTime™ Gene Expression Master Mix
PrimeTime™ One-Step 4X Broad-Range Master Mix
PrimeTime™ One-Step RT-qPCR Master Mix
rhAmp™ Genotyping Master Mix & Reporter Mixes
RNase H2 Enzyme
Oligo length standards
qPCR
PrimeTime™ Pathogen Research Panels
Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.
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Custom PCR Primers
rhPCR primers
RxnReady™ Primer Pools
PrimeTime™ qPCR Primer Assays
qPCR
PrimeTime™ Pathogen Research Panels
Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.
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ReadyMade Oligos
ReadyMade Primers
ReadyMade Random Hexamers
qPCR
PrimeTime™ Pathogen Research Panels
Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.
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qPCR
PrimeTime™ Pathogen Research Panels
Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.
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qPCR
PrimeTime™ Sexual Health Research Panels
Optimized sexual health panels remove sequence design guesswork—saving time and money with flexible fluorophore options, platform compatibility, and reliable results.
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CRISPR Gene Editing
CRISPR-Cas9 Systems
CRISPR-Cas12a (Cpf1) Systems
Guide RNAs & Libraries
Cas9 Guide RNAs
Cas12a Guide RNAs
Alt-R CRISPR-Cas9 Libraries
Custom Guide RNAs
CGMP Guide RNA Manufacturing
CRISPR
CRISPR-Cas9 Systems
Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.
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Cas Enzymes
Cas9 Enzymes
Cas12a Enzymes
GMP Enzymes
CRISPR
CRISPR-Cas9 Systems
Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.
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HDR Reagents
HDR Enhancer Protein
HDR Enhancer V2 (Small molecule)
HDR Donor Oligos
HDR Donor Blocks
CRISPR
CRISPR-Cas9 Systems
Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.
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rhAmpSeq™ CRISPR Data Analysis System
rhAmpSeq™ CRISPR Library Kit
rhAmpSeq™ CRISPR Panels
rhAmpSeq™ Index Primers
CRISPR
CRISPR-Cas9 Systems
Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.
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Genome Editing Detection Kit
CRISPR Off-target Analysis Services
CRISPR
CGMP gRNA Manufacturing
Accelerate your CRISPR therapeutics with our full CGMP guide RNA manufacturing/regulatory services.
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CRISPR
CRISPR-Cas9 Systems
Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.
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Gene Synthesis & Fragments
gBlocks & gBlocks HiFi Gene Fragments
eBlocks Gene Fragments
Gene Synthesis Service
Rapid Genes
Megamer ssDNA DNA Fragments
Genes
gBlocks HiFi Gene Fragments
Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.
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Genes
Rapid Genes
Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.
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Next Generation Sequencing
Library Preparation
DNA Library Prep Kits
RNA Library Prep Kits
Methyl-Seq Library Prep Kit
qPCR
Your Next MasterMix
Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.
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Hybridization Capture
xGen Predesigned Hyb Panels
xGen Custom Hyb Panels
xGen Hybridization Capture Core Reagents
qPCR
Your Next MasterMix
Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.
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Amplicon Sequencing
xGen Oncology Amplicon Panels
xGen Inherited Disease Amplicon Panels
xGen Infectious Disease Amplicon Panels
xGen Human Sample ID Amplicon Panel
xGen Custom Amplicon Panels
qPCR
Your Next MasterMix
Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.
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Library Normalization
xGen Normalase Module
xGen Pre-hybridization Capture Normalase Module
qPCR
Your Next MasterMix
Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.
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Anchored Multiplex PCR
RNA Assays
DNA Assays
ctDNA Panel
T & B Cell RNA Assays
Custom Assays
qPCR
Your Next MasterMix
Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.
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Archer Analysis
Adapters & Primers for NGS Sequencers
for Illumina Sequencers
for Ultima Genomics Sequencers
for Element Biosciences Sequencers
for DNBSEQ Sequencers
qPCR
Your Next MasterMix
Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.
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Sequencing Services
NGS
Custom Hyb Panels Design
We'll help you design fast, flexible target enrichment panels that fit into your NGS workflow.
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qPCR
Your Next MasterMix
Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.
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Gene Regulation
RNA interference
Antisense Oligonucleotides
miRNA inhibitors
Oligos
Antisense Oligonucleotides
Increase stability and reduce toxicity with nuclease-resistant mods and flexible chimeric designs.
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Reagents
DsiRNAs for RNAi
Effectively target cytoplasmic mRNA and noncoding RNA with predesigned or custom RNAi solutions.
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GMP Oligo Manufacturing
GMP Oligos
GMP Primers & Probes
MGB Eclipse® Probes
Oligos
Custom DNA Oligos
>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.
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CGMP CRISPR Manufacturing
CGMP Guide RNAs
CGMP Cas Nucleases
CRISPR Off-target Analysis Services
CRISPR
CGMP gRNA Manufacturing
Accelerate your CRISPR therapeutics with our full CGMP guide RNA manufacturing/regulatory services.
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CRISPR
CRISPR Off-target Analysis Services
Ensure the safety and efficacy of your CRISPR-based therapeutic with end-to-end services.
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OEM & Additional Services
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Oligos
Custom DNA Oligos
>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.
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Genes
Rapid Genes
Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.
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Explore Solutions
Applications
Cancer Research
Cancer whole genome sequencing
Cancer exome sequencing
NGS cancer screening
Biomarker discovery
TMB and MSI biomarkers
Cancer liquid biopsy
Minimal residual disease research
NGS
Cancer Research Solutions
High performance NGS solutions with flexible, efficient workflows for your research insights.
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Infectious Disease
COVID-19
Viral surveillance
NGS
Cancer Research Solutions
High performance NGS solutions with flexible, efficient workflows for your research insights.
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Rare & Inherited Genetic Disease
Antibody Discovery
Protein Engineering
NGS
Cancer Research Solutions
High performance NGS solutions with flexible, efficient workflows for your research insights.
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Resources
CRISPR Therapeutics eBook
Fundamental concepts, innovations, and insights about CRISPR R&D and the therapeutic landscape.
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Research Techniques
Genotyping
Gene Expression
CRISPR Genome Editing
Gene Knockout
Gene Knock-in
Gene Knockdown
Genes
gBlocks HiFi Gene Fragments
Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.
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Synthetic Biology
Cloning
Site Directed Mutagenesis
In Vitro Transcription
Genes
gBlocks HiFi Gene Fragments
Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.
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Next Generation Sequencing
Whole genome sequencing
Whole exome sequencing
Hybridization Capture
Amplicon Sequencing
Anchored Multiplex PCR Sequencing and Analysis
Targeted methyl sequencing
Whole transcriptome sequencing
Genes
gBlocks HiFi Gene Fragments
Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.
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Oligos
Custom DNA Oligos
>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.
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Genes
gBlocks HiFi Gene Fragments
Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.
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Oligos, PCR & qPCR
Digital PCR
rhAmp PCR
PCR Allele Competitive Extension (PACE) genotyping
SYBR Green dye assay and PrimeTime probe assays
Locked nucleic acids
Oligos
Custom DNA Oligos
>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.
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CRISPR
CRISPR workflow
CRISPR genome editing
CRISPR delivery
CRISPR
CRISPR Cas12a
Open up CRISPR editing to additional areas in genomes.
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Synthetic Biology
Antibody applications
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Resources
DNA Cloning Guide
Learn about common DNA cloning techniques, sequence design considerations, and troubleshooting tips.
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Tools
Oligo Design & Handling
OligoAnalyzer™ Tool
Analyzer definitions
Oligos
Custom DNA Oligos
>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.
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UNAFold Tool
Resuspension Calculator
Dilution Calculator
PrimerQuest Tool
Oligos
Custom DNA Oligos
>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.
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CRISPR Genome Editing
Alt-R Predesigned Cas9 Selection Tool
Alt-R Custom Cas9 Design Tool
CRISPR-Cas9 Design Checker
Alt-R CRISPR HDR Design Tool
rhAmpSeq Design Tool
rhAmpSeq™ CRISPR Data Analysis Tool
CRISPR
CRISPR Cas12a
Open up CRISPR editing to additional areas in genomes.
Learn More
CRISPR
CRISPR-Cas9 Systems
Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.
Learn More
qPCR Assay Design
PrimerQuest™ Tool
Realtime PCR Tool
PrimeTime™ Predesigned Gene Expression Assays
PrimeTime Multiplex Dye Selection
qPCR
Custom qPCR Probes
Multiplex your way with a wide range of high-efficiency quenchers, dyes, and scales.
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qPCR
Your Next MasterMix
Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.
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Gene Regulation and RNAi
Predesigned DsiRNA Selection Tool
RNAi Design Tool
Oligos
Antisense Oligonucleotides
Increase stability and reduce toxicity with nuclease-resistant mods and flexible chimeric designs.
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Reagents
DsiRNAs for RNAi
Effectively target cytoplasmic mRNA and noncoding RNA with predesigned or custom RNAi solutions.
Learn More
Genotyping
rhAmp Genotyping Design Tool
qPCR
Custom qPCR Probes
Multiplex your way with a wide range of high-efficiency quenchers, dyes, and scales.
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Genes & Gene Fragments
Codon Optimization Tool
gBlocks Gene Fragments Entry Tool
Genes & Gene Fragments order status
Genes
Rapid Genes
Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.
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NGS Tools
Library Concentration Conversion Calculator
Target Capture Probe Design & Ordering Tool
xGen™ Hyb Panel Design Tool
Custom Adapter Configurator Tool
NGS Solutions Builder Tool
Assay Marketplace
NGS
NGS Experts at Your Service
Reach your research goals faster with a consultation from our leading NGS specialists.
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qPCR
Your Next MasterMix
Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.
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A practical guide for PCR and qPCR primer design

A practical guide for PCR and qPCR primer design

Many factors can influence PCR experiments, including primer and probe characteristics such as location, length, interaction and self-folding, melting temperature, annealing temperature, and GC content. Review these general recommendations for designing primers and probes and for choosing target locations for PCR amplification.

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IDT’s commitment to biosecurity

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Explaining multiple peaks in qPCR melt curve analysis

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Expert tips for resuspending oligos and primers

Expert tips for resuspending oligos and primers

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Oligos, PCR, and qPCR

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