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Gene synthesis

This easy-to-use solution is an option for researchers who would prefer to skip in-house cloning steps and move quickly into functional studies with 100% sequence verified clonal DNA.

Ready to use. Ready for you.

Ordering

Complete synthetic genes with 100% sequence verification are provided in a plasmid cloning vector and ready to use in a variety of applications.

  • Guaranteed delivery dates
  • Easy online ordering and order tracking
  • No minimum order required or additional charges for custom vectors Include bullet as to whether this is RUO or EUA product

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* Gene sequences with added complexity can interfere with assembly and/or sequencing performance. Such sequences may result in less synthesis yield and/or additional services charges.

1 The time required to manufacture a gene is dependent on length, complexity, and vector choice. >85% orders are ready to ship within the number of business days indicated. Sequences that are unstable and/or toxic to E. coli can substantially affect delivery times.

Product details

Genes and MiniGenes™ are NGS-verified, circular double-stranded DNA in a plasmid. DNA sequences 25 bp to 5 kb are provided with IDT’s in-house cloning vectors or a custom plasmid vector of your choice (see Table 1) without additional cloning fees. Sequences greater than 5 kb can be reviewed for acceptance as a custom project. Plasmids are delivered dry and ready-to use with an accompanying QC report.

How to order Genes and MiniGenes

Single gene entry

  1. Under Ordering, click on the Order now button.
  2. Enter a name for your gene.
  3. Copy and paste, or enter your desired DNA sequence using A, C, G, and T bases only
    • Degenerate (mixed bases) or modified bases are not offered with our synthetic genes products.
    • Prior to adding to your order, sequence complexity can be tested using the Test Complexity button—this allows you to make simple changes to the sequence if necessary.
  4. Click the Add to Order button.
  5. Select a vector from the popup window.
  6. Complete the biohazard information and the order will be placed in the shopping cart for checkout.

Multiple gene entry

Under Ordering, click on the Order now button and select the Bulk Input option at the top left of the ordering tool. Separate your sequences by name as you import Excel or text files.

IDT cloning vectors

All our gene synthesis products are provided in an optimized cloning vector that is ready to be transformed into E. coli. To optimize manufacturing and delivery times, genes ≤5 kb in length are delivered in a “best-fit” vector (Table 1), for which you can choose between ampicillin or kanamycin selection markers. Additionally, we offer vectors that have been optimized for use with Golden Gate Assembly, as they are devoid of the most frequently used Type IIS restriction sites. Upon receiving your gene, you can then subclone it into the vector of your choice using a variety of methods. The identity of the cloning vector used, its sequence, and insertion site will be confirmed in the documentation that accompanies your product.

Table 1. Best-fit vectors for Genes ≤5000 bp

Vector nameVector sizeSelection markerApplicationSequence
pUCIDT (Amp)2752AmpicillinCloningpUCIDT (Amp).txt
pUCIDT (Amp) Golden Gate2752AmpicillinCloningpUCIDT (Amp) Golden Gate.txt
pUCIDT (Kan)2705KanamycinCloningpUCIDT (Kan).txt
pIDTSmart (Amp)2056AmpicillinCloningpIDTSmart (Amp).txt
pIDTSmart (Kan)1962KanamycinCloningpIDTSmart (Kan).txt

Product data

Sequence verification

All inserts for Genes and MiniGene products are sequence verified by NGS. Upon delivery of the plasmids a NGS-sequencing report, plasmid map, and FASTA file are available to you via your online IDT account.

Frequently asked questions

Biosecurity

Sequence Information is secure and confidential at IDT. Please see our Confidentiality Statement for more information. All online ordering steps, including sequence entry and your choice of parameters, are also secure and protected.

We screen the sequence of every gene fragment order we receive to (1) identify any regulated and other potentially dangerous pathogen sequences, and (2) verify that IDT’s gene customers are legitimate scientists engaged in beneficial research.

IDT is among the five founding members of the International Gene Synthesis Consortium (IGSC) and helped to create the IGSC’s Harmonized Screening Protocol. The Harmonized Screening Protocol describes the gene sequence and customer screening practices that IGSC member companies employ to prevent the misuse of synthetic genes. IDT takes the steps set out in the Harmonized Screening Protocol to screen the sequences of ordered genes and the prospective customers who submit those orders.

For more information about the IGSC and the Harmonized Screening Protocol, please visit the website at www.genesynthesisconsortium.org.

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In October 2010, the United States government issued final Screening Framework Guidance for Providers of Synthetic Double-Stranded DNA, describing how commercial providers of synthetic genes should perform gene sequence and customer screening. IDT and the other IGSC member companies supported the adoption of the Screening Framework Guidance, and IDT follows that Guidance in its application of the Harmonized Screening Protocol. For more information, please see 75 FR 62820 (Oct. 13, 2010), or https://federalregister.gov/a/2010-25728.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

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