Codon Optimization Tool

IDT codon optimization tool is designed to enhance sequence manufacturability.

The file contains the codon optimized sequence(s), you may upload it to the gene ordering portal for synthesis or order now via the “Order Item” button.

This tool screens for the following restriction enzyme sites:

For organisms not listed, a Custom Codon Table function is available under Optimization Type "Codon Optimization"

Expression Optimization: Context-sensitive algorithm-based codon selection aimed at replicating natural-like codon distribution profiles for the target species, prioritizes generating features associated with efficient protein expression.

Codon Optimization: Random codon selection based on codon usage frequency of the target species, includes Custom Codon Table and individual codon Manual Optimization functions, prioritizes minimizing manufacturing complexity.

Define custom restriction sites if needed. Restriction sites must be 5 - 15 valid characters.

Add

Toggle or add restriction sites to be avoided.

Provide additional 5' and/or 3' bases to be appended to the ends of the optimized sequence. ATCG characters only. Flanking sequences will not be codon optimized.