Loading Tool...
Codon Optimization Tool
IDT codon optimization tool is designed to enhance sequence manufacturability.
Download
The file contains the codon optimized sequence(s), you may upload it to the gene ordering portal for synthesis or order now via the “Order Item” button.
Restriction Enzymes
This tool screens for the following restriction enzyme sites:
Configuring Restriction Enzymes
Define custom restriction sites if needed.
Restriction sites must be 5 - 15 valid characters.
Toggle or add restriction sites to be avoided.
Configuring 5' and 3' Bases
Provide additional 5' and/or 3' bases to be appended to the ends of the optimized sequence.
ATCG characters only. Flanking sequences will not be codon optimized.
5' prefix
3' suffix
Selected:
5' Nucleotides:
3' Nucleotides:
Please use the "Standard" format provided for tables in the kazusa database.
Fields: [triplet] [amino acid] [fraction] [frequency: per thousand] ([number])
Enter items as shown below. Name and sequence are required.
To optimize a portion of the sequence, enter start and end locations after the sequence. Start and end locations are inclusive.
To optimize the whole sequence, leave the start and end locations blank.
Reading frame selection not available for FASTA entry. Amino acid sequences are entered using single-letter abbreviations.
To optimize a portion of the sequence, enter start and end locations after the sequence. Start and end locations are inclusive.
To optimize the whole sequence, leave the start and end locations blank.
Reading frame selection not available for FASTA entry. Amino acid sequences are entered using single-letter abbreviations.
Results
Sequence passes initial screening.
Total Complexity Score:
Complexity Description
Score
Manual Optimization
Manual optimization allows you to manually select specific codons at designated positions, giving you full control over codon usage in your sequence