Affinity Plus DNA & RNA Oligonucleotides

Affinity Plus DNA & RNA Oligonucleotides are LNA single- and double-stranded sequences. With the Oligo Entry ordering tool, you can design the sequence you require with 1 to 20 LNA nucleotides to suit your specific research needs.

Every Affinity Plus DNA & RNA Oligo you receive is deprotected and desalted to remove small molecule impurities. In addition, your oligos will be sequence verified via proprietary ESI mass spectrometry methods*, and quantified twice by UV spectrophotometry to provide accurate yield measurements.

*With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Using the Oligo Entry tool

To enter individual Affinity Plus LNA nucleotides, insert "+" before the base (+A, +C, +G, +T).

Enhanced oligo stability

Affinity Plus Oligonucleotides provide identical annealing properties as other manufacturers’ LNA sequences (Figure 1). The increased hybridization affinity and thus hybridization melting temperature (T_m) means enhanced sequence stability both in vitro and in vivo.

Sensitive target detection in qPCR and SNP assays

The Affinity Plus LNA sequences can be used as probes, for example, in qPCR and genotyping assays. The higher T_m of these sequences provides better hybridization stability to target regions, especially those with low GC content. This increased stability also makes it possible to use shorter probe designs, which are helpful when target regions are limited in size. For these applications, obtain these LNA probes as Affinity Plus qPCR Probes. These LNA probes provide the same sensitive amplification as other LNA probes, and show increased sensitivity over unmodified probes (see Figure 2 on the Performance tab of the Affinity Plus qPCR Probes product page).

Brochures and flyers

Frequently asked questions