In addition to targeted sequencing, IDT has library prep kits and NGS adapters available to support the workflows for high throughput sequencing. IDT library prep kits produce quality libraries from a range of sample input amounts. Unique molecular identifier (UMI) sequences can be incorporated during ligation enable a variety of deduplication and error correction strategies. Our range of adapter offerings contain a variety of indexing options including unique dual indexes (UDIs), which mitigate sample misassignment due to index hopping. These workflow components are compatible with our targeted sequencing offerings or can fit into workflows for other next generation sequencing applications.
Hybridization capture is a targeted next generation sequencing method that uses long, biotinylated oligonucleotide baits (probes) to hybridize to the regions of interest. It is particularly helpful when genotyping, detecting rare variants, and exome sequencing. xGen hybridization capture panels consist of 5′ biotin–modified oligonucleotide probes that are individually synthesized. Individual probes are made in large lots and then aliquoted to maintain reproducibility. This rigorous manufacturing process gives xGen hybridization capture panels a unique advantage over array-derived pools, in which missing or truncated probes cannot be identified before sequencing. Using IDT proprietary synthesis methods, even probes with high GC and AT content are appropriately represented. In addition to providing stocked hyb capture panels, we can design custom panels for a given experiment. xGen Blocking Oligos and the xGen Hybridization and Wash Kit round out the workflow. We work with you to tailor products to your needs.
Our proprietary rhAmp PCR technology drives the rhAmpSeq amplicon sequencing system. Using RNA-base–containing blocked primers (rhAmp primers), this technology harnesses the intrinsic properties of the RNase H2 enzyme to recognize and cleave DNA:RNA duplexes. rhAmp PCR is thus a powerful tool for increasing amplification specificity and minimizing the biggest limitation of PCR multiplexing—primer dimers.
The rhAmpSeq system combines the performance advantages of rhAmp PCR with a fast, easy-to-use workflow that requires only 2 PCR amplification steps to generate amplicon libraries for Illumina sequencing platforms. Coupled with our best-in-class DNA and RNA manufacturing, the rhAmpSeq system offers high-quality, targeted, sequencing results quickly and cost-effectively. Use the rhAmpSeq Sample ID Panel for easy, reliable human sample identification or design your own with the rhAmpSeq Design Tool.
Comprehensive offering of custom and catalog adapters for any NGS platform from the leader in nucleic acid manufacturing. Choose from a variety of barcoding and error correcting strategies, including designs with unique dual index (UDI) barcodes and unique molecular indentifiers (UMIs).
From the Lotus DNA Library Prep Kit’s flexible workflow to the xGen Prism DNA Library Prep’s superior performance with difficult samples, we have the right kit for your application.
A suite of high performing enrichment probes, blockers, and workflow reagents for targeted sequencing. The xGen product portfolio enables unparalleled target coverage and uniformity, even across difficult genomic regions containing high GC content.
The rhAmpSeq system enables highly accurate amplicon sequencing on Illumina® NGS platforms. Whether you are investigating thousands of targets or a few, the fast and easy rhAmpSeq workflow generates NGS-ready amplicon libraries for deep, targeted resequencing.
Customized products and services to meet the rigorous quality requirements for clinical and molecular diagnostic applications.
IDT and Illumina partner for whole exome sequencing.