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HotPlates™ DNA Oligos

Custom DNA oligos in plates, delivered in 2 business days

Hot Plates DNA Oligos are standard, desalted DNA oligos delivered in V-bottom plates. Place your order before the specified deadline to receive your plates in 2 business days.

Ordering

  • Receive your custom HotPlate DNA Oligos 2 business days after placing your order
  • Each oligo is verified by ESI mass spectrometry* expedited without compromising quality
  • Every oligo normalized to your specifications-let us do the formulation for you
  • No maximum on number of plates

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

HotPlates Oligos

Delivered dry in 96-well, V-bottom plates. A minimum of 48 oligos is required per plate.* To meet the ordering deadline, orders for HotPlates Oligos must be placed online.

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* The following service fee will be added to each plate:

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Product Details

HotPlates DNA Oligos are standard, desalted DNA oligos delivered in V-bottom plates and shipped by our fastest method. If you place your order before the day’s deadline (below), you will receive your plates in 2 business days.

Every DNA Oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled via proprietary ESI mass spectrometry methods*, and quantified twice by UV spectrophotometry for accurate yield measurements.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Specifications

  • Normalized to 2–10 nmol per well or provided at full synthesis yield
  • 15–60 DNA bases; no modifications or modified bases
  • Deprotected and desalted
  • Each oligo quality controlled by ESI mass spectrometry*
  • Delivered dry in 96-well V-bottom plates
  • Minimum of 48 oligos per plate
  • Available in US, Canada, and Europe

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Ordering deadlines

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US and Canada

Order online by 3:00 pm ET

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Europe

Order online by 1:00 pm CET

Product Data

Quality

IDT’s long-standing reputation as a leader and pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that ensure the fastest turnaround times possible. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to easily monitor and refine instruments and reagents with ease. Our ability to control these variables allows us to produce oligos that are unmatched in quality and consistency.

An important measure of quality for any oligo manufacturer is coupling efficiency, because this directly affects the amount of full-length product you receive. Our manufacturing processes achieve extremely high coupling efficiencies (Figure 1) and are standardized across our global manufacturing sites (Figure 2) to provide you with most consistent quality in the industry. No matter where in the world you are conducting your research, an IDT oligo is an IDT oligo. Our commitment to quality and consistency results in an extremely reliable product that will perform the same each time it is used. Because your research requires a steady supply of oligos over time, this reliability provides confidence in your research.

Figure 1. IDT proprietary platforms have a better coupling efficiency than other suppliers, which provides more full-length oligonucleotides in your order. Small increases in coupling efficiency (≤1%) result in measurable increases in full-length product yield. The curves represent the theoretical yield for different lengths of oligos based on a coupling efficiency of 99.4% (IDT oligos, n = 126) and 99.1% (other supplier, n = 134 from three different suppliers) using the formula, percent full length product = (eff )(n-1)*100 where eff = coupling efficiency (for example, 99.4% = 0.994) and (n–1) is the number of coupling reactions needed to make an oligo of length n.

Figure 2. IDT tracks coupling efficiency at our manufacturing sites to ensure consistent quality. Many variables can lead to reduced oligo quality, including ambient temperature, humidity, and input reagents. Monitoring of coupling efficiency at IDT manufacturing sites ensures that we provide our customers with high quality products. A quarterly average is shown (n = 16 per manufacturing site).

Resources

Frequently asked questions

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

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