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Rapid HPLC™ DNA Oligos

HPLC purified oligos offer speed without comprising the quality of your oligos. Each Rapid HPLC DNA Oligo is quality controlled by electrospray ionization-mass spectrometry and capillary electrophoresis or analytical HPLC.

Ordering

Rapid HPLC Oligos

Delivered dry in tubes. To meet the ordering deadline, orders for Rapid HPLC Oligos must be placed online.

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The Rapid HPLC fee for expedited HPLC purification will be applied for each oligo.

Product Details

HPLC purification

High performance liquid chromatography (HPLC) purification, a form of column chromatography, can be performed in one of two ways. Reverse-phase (RP) HPLC separates oligos based on their affinity for particular solvents, while ion-exchange (IE) HPLC separates oligonucleotides based on their charge. The type of HPLC we use is dependent on the sequence and molecular characteristics of the oligo. HPLC-purified oligos experience an unavoidable loss of mass, but this is offset by the dramatic increase in purity. To learn more about our purification services, click here.

Rapid HPLC Oligos are purified using the same equipment and protocols as standard HPLC purification. The resulting purity is >85% full-length product. All oligos are quality control checked by electrospray ionization—mass spectrometry (ESI-MS), with traces available for free on our website. If you place your order before the day’s deadline (below), your Rapid HPLC Oligos will be shipped on the next business day.

Specifications

  • Purified to contain >85% full-length product
  • 10–60 bases; available with 5′ Phosphorylation or 5′ Amino Modifier C6
  • Each oligo is quality controlled by with ESI-MS
  • Shipped dry in tubes
  • Available in the US and Canada

Ordering deadlines

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Available in US and Canada only.

Order online by 3:00 pm ET

Product Data

IDT’s long-standing reputation as a leader and pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that ensure the fastest turnaround times possible. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to easily monitor and refine instruments and reagents with ease. Our ability to control these variables allows us to produce oligos that are unmatched in quality and consistency.

An important measure of quality for any oligo manufacturer is coupling efficiency, as this directly affects the amount of full-length product you receive. Our manufacturing methods allow us to achieve extremely high coupling efficiencies (Figure 1). This commitment to quality results in our ability to provide extremely reliable products.

IDT proprietary platforms have a better coupling efficiency than other suppliers, which provides more full-length oligonucleotides in your order.

Figure 1. IDT proprietary platforms have a better coupling efficiency than other suppliers, which provides more full-length oligonucleotides in your order. Small increases in coupling efficiency (≤1%) result in measurable increases in full-length product yield. The curves represent the theoretical yield for different lengths of oligos based on a coupling efficiency of 99.4% (IDT oligos, n = 126) and 99.1% (other supplier, n = 134 from three different suppliers) using the formula, percent full length product = (eff )(n-1)*100 where eff = coupling efficiency (for example, 99.4% = 0.994) and (n–1) is the number of coupling reactions needed to make an oligo of length n.

Resources

Frequently asked questions

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

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