gBlocks Gene Fragments
gBlocks Gene Fragments are double-stranded DNA fragments 125–3000 bp in length with a median error rate of less than 1:5000. They are manufactured with the same industry-leading, high-fidelity synthesis chemistries that were developed for our
Ultramer™ DNA Oligos.
Each gBlocks Gene Fragment goes through a quality control process, which includes size verification by capillary electrophoresis and sequence identification by mass spectrometry. This rigorous testing that most recombinant colonies obtained from cloning
each gBlocks Gene Fragment will contain the desired insert. More complex sequences may need the end user to sequence additional clones.
gBlocks HiFi Gene Fragments
gBlocks HiFi Gene Fragments are double-stranded DNA fragments between 1000–3000 bp that are sequence-verified via NGS with a median error rate of less than 1:12,000. These high-quality, high-fidelity fragments facilitate the assembly of
large and complex sequences, matching both the length and accuracy needed to minimize the introduction of unwanted substitution or deletion errors.
With either gBlocks or gBlocks HiFi Gene Fragments, you can easily assemble and clone your DNA fragment into the vector of your choice using a variety of cloning methods. For added flexibility, you can order gBlocks Gene Fragments with
or without a 5′‑phosphate group.
gBlocks Gene Fragment Libraries
gBlocks Gene Fragment Libraries are pooled gBlocks fragments of 251–500 bp in total length. gBlocks Gene Fragment Libraries are ideal for generating recombinant antibodies or for protein engineering, allowing researchers to generate hundreds
of thousands of constructs within a reasonable budget. The variable regions can be up to 18 consecutive N (any base) or K (G or T) bases long and must be at least 125 bp from either end of the gene fragment (Figure 1).