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Double-stranded DNA fragments

Design, build, explore. Go.

Using synthetic, double-stranded DNA fragments will save you time and money compared to traditional cloning methods. We offer a variety of gene fragments, ranging from eBlocks™ Gene Fragments for high-throughput antibody research to gBlocks™ HiFi Gene Fragments for large gene construction. We have quick and reliable solutions to meet your experimental needs.

  • The trusted industry standard for purity and quality
  • Adaptable and flexible to fit your workflow and budget
  • Dependably fast delivery
Icons_Ocean_85x85_Design
Design
Icons_Ocean_85x85_Build-Assemble
Build
Icons_Ocean_85x85_Verification-Validation
Test

Use Table 1 to help choose the best double-stranded DNA product for your experiment.

Table 1. Comparison of IDT gene fragments.

 eBlocks Gene FragmentsgBlocks Gene FragmentsgBlocks HiFi Gene Fragments
Length300–900 bp125–3000 bp1000–3000 bp

Turnaround time (BD)*
   Tubes
   Plates

 
N/A
1–3

 
2–8
10–15

 
6–10
10–15

Minimum order
   Tubes
   Plates

 
N/A
24 fragments

 
1 fragment
24 fragments

 
1 fragment
24 fragments

Yield200 ng250–1000 ng1000 ng
QC†CECE/MSNGS
FormatPlateTube/PlateTube/Plate
Recommended applicationsAntibody discovery
Vaccine development
High throughput screening		Gene construction
qPCR/PCR standards
NGS controls		Large gene construction,
including pathway development and microbe design

* BD = business days; N/A = not applicable (eBlocks fragments are not available in tubes)

† CE = capillary electrophoresis; MS = mass spectrometry; NGS = next generation sequencing


Find your correct clone with minimal screening

Using IDT gene fragments will reduce the time and expense of screening colonies compared to fragments from other suppliers (Table 2). Cloning efficiency is affected by many factors, including the cloning method used, the stability of the cell line and plasmid, vector preparation, and toxicity or stress from expression of coding sequences. The values in Table 2 represent typical screening numbers needed when using a seamless assembly method, such as Gibson Assembly® (Synthetic Genomics) or NEBuilder® HiFi assembly (New England BioLabs), and when issues mentioned above are not significant contributors to error or selection.

Table 2. Approximate number of colonies to screen for a 90% chance of getting a correct clone.

Sequence lengtheBlocks Gene FragmentsgBlocks Gene FragmentsgBlocks HiFi Gene FragmentsOther supplier
50033N/A*5
9004
1500N/A*27
2000410
2500No data available
3000

* N/A = not applicable (IDT gene fragments are not available at the lengths indicated)