Large-scale oligo synthesis—Normalized Oligos

Overview

Manufacturing capabilities

Numerous collaborations with our customers have enabled us to optimize our manufacturing processes and platforms for bulk synthesis of various types of oligos. Our large-scale oligo synthesis capabilities range from standard, unmodified oligos to highly complex oligos for high-throughput, and other commercial applications. You can acquire milligram to gram quantities of highly pure nucleic acids, including aptamers, ribozymes, miRNAs and siRNAs, next generation sequencing adapters, PCR primers, and dual-labeled probes.

Additional features of our large-scale offerings include:

• Final yields from 10 mg to 10 g
• Standard DNA and RNA bases, modified bases, chemical modifications, linkers, and fluorophores
• Unmodified DNA lengths between 4−100 bases
• Unmodified RNA lengths between 4−70 bases
• GMP manufacturing available for customers requiring controlled processes and traceability

Application-specific solutions

• CRISPR Libraries: In pooled CRISPR screens, each guide RNA (gRNA) targets a different gene. If some guides are overrepresented, it can skew the results. Large-scale oligo normalization ensures equal representation of each gRNA, leading to more reliable gene knockout data and better statistical power.
• Next-Generation Sequencing: In amplicon or hybrid capture-based sequencing, uneven oligo concentrations can lead to biased coverage. Bulk oligo normalization helps achieve uniform read depth across targets, improving variant identification and data quality.
• Gene synthesis or assembly: Uneven oligo concentrations can cause assembly errors or missing fragments. Large quantities of normalized oligos ensure complete gene assembly, especially in multiplexed reactions.

GMP

At our lab, quality isn’t just a checkbox—it’s a commitment. Our GMP products and services are customized to your exact specifications with the documentation required for components used in commercial and molecular applications.

• ISO 13485:2016
  IDT’s GMP products and services are manufactured under a quality management system that complies with ISO 13485:2016. The scope of this certification includes manufacturing/processing services, customer support, sales/distribution for custom, synthetic nucleic acid and associated reagent/consumable products for molecular biology applications, including (but not limited to) qPCR, sequencing, synthetic biology and functional genomics as a material for molecular in-vitro diagnostic devices and laboratory developed tests (BSI FM 513219).
• ISO 9001:2015
  IDT operates a quality management system that complies with ISO 9001:2015 (BSI FM 88954). This certification represents our robust system for designing, developing, and producing oligo-based tools for life science research. It ensures our processes are efficient, consistent, and continuously improving.

Whether you're doing basic research or developing diagnostic tests, you can count on our large-scale oligo synthesis to be consistent, well-documented, and backed by rigorous quality control. We even provide certificates of analysis for full traceability. Learn more about our GMP capabilities here.

Additional services

In addition to using industry-leading oligos, when you work with our team of large-scale experts you will benefit from a variety of personalized services.

These include:

• Confidential, application-specific guidance for your transition from small- to large-scale production
• Flexible ordering methods including online ordering availability
• Custom purification options and purity specifications
• Formulation services for the creation of oligo pools, primer mixes, assays, and duplexes
• Custom vialing and plating options with consistent or variable fill sizes
• Verification and release of product compliance before shipment
• Hard-copy analytical QC documentation and certificates of analysis

Learn more about these individual services here.

GMP

Our GMP products and services are customized to your exact specifications with the documentation required for components used in commercial and molecular applications. Learn more about our GMP capabilities here.