Processing
CRISPR genome editing
CRISPR knock-out: Tips and tricks
For CRISPR gene knock-outs, it’s important to design the right guide RNA to increase genome editing efficiency. For a successful knock-out, the Cas nuclease makes a double strand break (DSB) which is repaired through the non-homologous end joining pathway (NHEJ). Read more about the tips and tricks you need to know to increase the efficiency of your CRISPR experiments.
- CRISPR knock-out: Tips and tricks
- Navigating the regulatory landscape: A business perspective on cell and gene therapy approvals
- Successful CRISPR genome editing in hard-to-transfect cells (i.e., Jurkat cells)
- CRISPR-Cas nucleases: Understanding PAM requirements and Cas diversification strategies
- Tackling sickle cell disease with precise and efficient gene editing
- How to use CRISPR-Cas9 technology to generate mice models for research
- Importance of the PAM sequences in CRISPR-Cas9 gene editing
- HDR and Cas9 nickase design: What you should know
- CRISPR-Cas9: What are the pros and cons?
- Primer design and other tools you should know about
- Single base editing
- Customizing CAR-T cells using the CRISPR-Cas9 system
- CRISPR knock-in: Tips and tricks
- CRISPRa and CRISPRi: Similarities and differences you should know about
- Megamer single-stranded donor templates (ssDNA or ssODNs) for successful homology-directed repair (HDR) in genome editing applications
- The CRISPR Basics Handbook: An IDT publication to help you learn CRISPR
- Genome editing tip: A CRISPR RNA annealing step can increase editing efficiency
- CRISPR GUARD: A short gRNA-based system that facilitates precise genome edits
- Alt-R™ HDR Donor Blocks can enable long DNA knock-ins while decreasing undesired edits
- Improving efficiency of homology-directed repair (HDR)
- CRISPECTOR, a statistical CRISPR genome editing analysis software for research
- A.s. Cas12a Ultra, a highly effective CRISPR genome editing enzyme
- A fusion gene identification strategy using CRISPR-Cas9 and long read nanopore sequencing
- Genome Oligopaint: Painting a clearer picture with oPools™ Oligo Pools
- Analyze your CRISPR genome edits with IDT's cloud-based software
- CRISPR-based depletion of unwanted sequences improves targeted next generation sequencing
- Applications and benefits of CRISPR functional genomics screens using arrayed guide RNA libraries
- SHERLOCK, PAC-MAN, and more: How CRISPR-Cas13 enzymes may be useful in the COVID-19 pandemic and beyond
- Stability of CRISPR reagents under different freezing, storage, and thawing conditions
- Consider ribonucleoprotein (RNP) instead of CRISPR plasmids!
