Charité/Berlin (WHO) protocol primer and probe panel

On January 23, 2020 collaborators from Charité – Universitätsmedizin Berlin Institute of Virology, German Centre for Infection Research, Tib-Molbiol, Erasmus MC, Rotterdam, and many other entities designed an assay and published a RT-PCR protocol for the detection of 2019-nCoV (now known as SARS-CoV-2) [1].  "The Berlin Panel” detects the RdRp and E genes to positively identify SARS-CoV-2.  IDT provides primers and probes for this assay for the detection of the virus.  Primers and probes are manufactured at IDT under ISO 13485:2016.  The kits include primers and 5’ FAM - ZEN™/Iowa Black® FQ probes.

 

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SARS-CoV-2 (2019-nCoV) Charité/Berlin Primer Probe Panel

  • Manufactured in a certified, template-free environment under ISO 13485:2016
  • Functionally validated and confirmed template-free to cycle 45 by NTC testing
  • Next-day shipping

 

 

SARS-CoV-2 (2019-nCoV) Charité/Berlin Primers & Probes

  • Manufactured in a certified, template-free environment under ISO 13485:2016
  • Functionally validated and confirmed template-free to cycle 45 by NTC testing
  • Next-day shipping

 

 

SARS-CoV-2 (2019-nCoV) Charité/Berlin RUO Plasmid Controls

  • NGS sequence-verified
  • Next-day shipping

2019-nCoV_RdRp (ORF1ab) control: Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome (GenBank: NC_045512.2) 

2019-nCoV_E control: Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome (GenBank: NC_045512.2)

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References

  1. (2020) Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. [Online] Eurosurveillance. [Accessed 8 Apr 2020].