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CDC assays for SARS-CoV-2 detection

The US Centers for Disease Control and Prevention (CDC) has designed RT-PCR assays and published a protocol for the detection of the 2019-nCoV. IDT provides primers and probes for these CDC assays for the identification of virus. The primers and probes are manufactured at IDT under ISO 13485:2016 conditions and are available for research use only (RUO). The kits include primers and 5’ FAM / ZEN™ / 3’ Iowa Black™ FQ probes. 2019-nCoV controls* are available separately.

On February 4th, 2020, the FDA issued an Emergency Use Authorization (EUA) to the CDC to expand the use of assays to positively identify the coronavirus [1.2].

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SARS-CoV-2 (2019-nCoV) CDC qPCR Probe Assay

  • Manufactured in a certified, template-free environment under ISO 13485:2016
  • Functionally validated and confirmed template-free to cycle 45 by NTC testing
  • Next-day shipping

Updated October 2, 2020

The CDC has quality controlled and authorized primers and probes for the N1, N2 and RP assays from following batches to be sold under the CDC EUA (dated February 29, 2020, and as amended):

0000500383, 0000504847, 0000505594, 0000505969, 0000507295, 0000507509, 0000508150, 0000508785, 0000509402, 0000510344, 0000510908, 0000511393, 0000512209, 0000512692, 0000513132, 0000522562, 0000524162, 0000527162, 0000529986, 0000536597, 0000538650, 0000540655, 0000542159 & 0000550657.

The CDC did not QC the N3 assays, and the N3 assay is not part of the authorized kits. The FDA approved the CDC’s amended protocol that excludes the N3 Assay on March 15, 2020. All kits from all future batches of IDT primers and probes will not include the N3 assay.

SARS-CoV-2 (2019-nCoV) CDC RUO Primers and Probes

  • Manufactured in a certified, template-free environment under ISO 13485:2016
  • Functionally validated and confirmed template-free to cycle 45 by NTC testing
  • Next-day shipping

 

SARS-CoV-2 (2019-nCoV) CDC RUO Plasmid Controls

  • NGS sequence-verified
  • Next-day shipping

    nCoV-N control: Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome (GenBank: NC_045512.2)

    MERS control: Middle East respiratory syndrome-related coronavirus isolate KNIH/002_05_2015, complete genome (​GenBank: MK796425.1​)

    SARS control:  B​at SARS-like coronavirus isolate bat-SL-CoVZC45, complete genome (GenBank: MG772933.1)


    * The IDT controls have not been designed, tested, validated by CDC, nor are they in any way endorsed by the CDC or any other public health agency. These controls were independently designed by IDT against the entire nucleocapsid gene of SARS, MERS, and 2019-nCOV. These controls are offered as a convenience to our customers. Controls should be validated by the end-user if used for the interpretation of test results.

    Don’t see what you need for your nCoV experiments?

    We can accommodate custom requests.

    Contact us

    References

    1. (2020) Emergency use authorization. [Online] US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
    2. (2020) CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel . [Online] US Centers for Disease Control and Prevention. [Accessed 3 Mar 2020].