Welcome to the IDT family!
Your product is now available from Integrated DNA Technologies.
Many of the Swift products you have grown to love are now part of our new complete portfolio, xGen™ NGS. Through this new partnership we are pleased to offer you comprehensive next generation sequencing solutions.
xGen NGS—made for you.
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Welcome to the IDT family!
Find Archer now at IDT!
All Archer information is now available on IDT’s website. You can view Archer assays alongside IDT’s xGen™ NGS portfolio to find the best next generation sequencing solution for your lab.
Confidently detect more with Archer NGS assay solutions for your solid tumor, blood cancer, immune profiling, and genetic disease research.
Processing
Understanding melt curves for improved SYBR Green assay analysis and troubleshooting
qPCR assays using intercalating dyes, such as SYBR Green dye, are an economical and effective tool for measuring gene expression. To interpret intercalating dye assays, users need to know how to analyze melt curves, and understand the benefits and limitations of such analysis. In this presentation, Nick Downey, PhD, covers melt curve basics and shares examples of multiple peaks due to suboptimal sample prep, primer dimers, and asymmetric GC content of amplicons. He also discusses troubleshooting strategies. Experienced and novice users will benefit from an overview of uMelt software, developed by the Wittwer lab at the University of Utah, that can predict the melt profile of your assay before you run your experiment.
Published on: April 03, 2015