{"id":2783,"date":"2024-11-18T17:16:37","date_gmt":"2024-11-18T17:16:37","guid":{"rendered":"https:\/\/devpages.idtdna.com\/page\/how-to-resuspend-store-and-improve-the-longevity-of-your-idt-gene-fragments"},"modified":"2025-08-08T06:50:19","modified_gmt":"2025-08-08T06:50:19","slug":"how-to-resuspend-store-and-improve-the-longevity-of-your-idt-gene-fragments","status":"publish","type":"post","link":"https:\/\/sgstage.idtdna.com\/page\/support-and-education\/decoded-plus\/how-to-resuspend-store-and-improve-the-longevity-of-your-idt-gene-fragments\/","title":{"rendered":"How to resuspend, store, and improve the longevity of your IDT Gene Fragments"},"content":{"rendered":"

How to resuspend eBlocks and gBlocks Gene Fragments<\/h2>\n

Standard gBlocks Gene Fragments <\/a> and gBlocks HiFi Gene Fragments<\/a> are dried down and shipped in tubes (unless otherwise requested to ship in plates or ship wet). Plate products, including gBlocks and eBlocks Gene Fragments, are shipped on dry ice, if wet and ambient, if dry. Once you receive your DNA fragments, it’s recommended to perform IDT’s resuspension protocol. gBlocks Gene Fragments<\/a> are stable for up to two years while dried and stored at −20C.<\/p>\n

Follow the steps below when resuspending your DNA fragments:<\/p>\n

1. Prepare your DNA fragment:<\/strong> Once you receive your DNA fragments, spin down the tube in a microcentrifuge for 5 seconds. This ensures that the DNA pellet is at the bottom.  Since the pellet can be statically charged or lodged in the cap during shipping, doing this step prevents the pellet from flying out of the tube or remaining in the cap thus losing yield.<\/p>\n

2. Add molecular grade water or buffer<\/strong>:  After centrifugation, resuspend the pellet in molecular-grade water or TE buffer (pH 7.5–8) <\/a> the required concentration. We recommend you use either a molecular grade water or a buffer such as IDTE, pH 8, to achieve a final concentration of 10 ng\/µL. Please note that concentrations <1 ng\/µL can result in material loss due to adherence to the plastic tube.<\/p>\n

3. Vortex briefly: <\/strong>Mix the solution briefly using a vortex.<\/p>\n

4. Heat the tube:<\/strong> Incubate at approximately 50°C for 15–20 minutes. Heating ensures the solvent contacts the entire pellet, even if it is stuck to the side of the tube, thereby increasing the likelihood of complete resuspension.<\/p>\n

5. Vortex and centrifuge<\/strong>: After heating, briefly vortex again and then centrifuge.<\/p>\n

6. Measure final concentration:<\/strong> Verify that the concentration of the resuspended gBlocks Gene Fragments to ensure that the full product has gone into solution. Use Nanodrop quantification to verify the final concentration. This method is preferred for short gBlocks Gene Fragments, as the Qubit™ system may give artificially low readings[1]<\/a>.<\/p>\n

How to store eBlocks and gBlocks Gene Fragments<\/h2>\n

IDT Gene Fragments are stable for up to two years under proper storage conditions.<\/p>\n

Here are some recommendations for improving the preservation of your gBlocks Gene Fragments:<\/p>\n