Processing
Synthetic gene design made easy
The Codon Optimization Tool converts the DNA or protein sequence from one organism for expression in another. The IDT algorithm provides the best sequence option by screening and filtering sequences to lower complexity and minimize secondary structures.
- Rebalance codon usage
- Decrease sequence complexity
- Avoid rare codons
Optimize and order in minutes
Get to work as quickly as possible and spend less time troubleshooting your expression experiments. This Codon Optimization Tool is integrated with our complexity checker and API Sci Tools for a seamless design and ordering experience.
- Easily input DNA or amino acid sequences
- Optimize hundreds of sequences in seconds
- Choose manual optimization for full control over the process
Expert support and customizable options
- PhD-level, gene synthesis specialists are available to provide additional guidance with your design
- Optimization based on your custom codon table
- Customization options offered for CAI targeting, motif avoidance or inclusion, and structural avoidance
Resources
Citations
- Sebesta J and Peebles C. (2020) Improving heterologous protein expression in Synechocystis sp. PCC 6803 for alpha-bisabolene production. Metab Eng Commun 10:e00117.
- Razeq FM, Jurak E, et al. (2018) A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans. Biotechnol Biofuels 11:74.
- Addepalli B, Lesner NP, Limbach PA. (2015) Detection of RNA nucleoside modifications with the uridine-specific ribonuclease MC1 from Momordica charantia. RNA 21:1746–1756.
- Swainsbury DJK, Martin EC, et al. (2017) Engineering of a calcium-ion binding site into the RC-LH1-PufX complex of Rhodobacter sphaeroides to enable ion-dependent spectral red shifting. Biochem Biophys Acta 1858(11):927–938.
Need help or inspiration?
If you need further assistance, please contact our Genes Support group at genes@idtdna.com.