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Cancer Research Sequencing

Overview

Most cancers start with mutated genes. Gene mutations can be inherited or can occur from environmental exposure. Studying genomics, genes, and gene function gives researchers insight into how mutated genes impact cancer symptoms, tumor progression, and health outcomes.

What causes cancer?

Most cancers are not purely hereditary or only caused by acquired mutations. Many types of cancers are caused by a combination of heredity and the environment. For example, you have an increased risk for skin cancer if you have family history of skin cancer, but if you never go in the sun, you will be much less likely to develop skin cancer. The mutations acquired from environmental exposure are called somatic mutations. They cannot be passed on to the effected person’s descendants. Somatic mutations differ from germline mutations, which are inherited. You may inherit germline mutations from your parents which increase your risk for skin cancer. When you go in the sun, you can acquire somatic mutations that increase your total mutation load, which is the combination of all somatic and germline mutations. Mutation load can be determined by sequencing, such as  whole exome sequencing, and can be a powerful predictor of a person’s risk for developing cancer.

Cancer research

There are two main areas of focus for cancer research: prevention and treatment. Understanding how cancer develops can help researchers find methods for both areas. Cancer research is furthered by cancer genomics research. Genomics research can reveal biomarkers or genes that indicate risk. Cancer treatment research has two approaches: tumor profiling and mutation load. A patient’s genotype can provide insight into how a patient might respond to a specific cancer therapy. If the person’s cancer is at an advanced stage, their tumor may have developed some of its own somatic mutations, which can also affect how a patient might respond to treatment. Tumor profiling is performed using either whole genome sequencing, or whole genome sequencing of tumor samples to identify mutations in the tumor. Tumor-normal profiling compares the genetic profile of tumor samples to the genetic profile of healthy tissue samples.

Cancer sequencing methods

Next generation sequencing (NGS) is a massively parallel sequencing technology that can provide valuable insight into cancer genetics. There are several approaches to using this technology for cancer research. Whole genome sequencing provides a comprehensive view of the genetic sequence of the sample, whether it be blood, tissue, or tumor. Targeted next generation sequencing focuses on specific areas of the genome that might be more relevant for cancer. For example, whole exome sequencing provides insight into protein-coding genes and is especially useful when evaluating mutation load. Hybridization capture panels can be customized to include non-coding genes that might be relevant to a specific cancer, e.g., leukemia or breast cancer. NGS can include sequencing DNA or RNA. Because RNA defines the way genes are expressed, RNA sequencing can also help researchers learn more about cancer as it can help isolate unique transcripts and indicate gene expression profiles.

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Hand-picked information on targeted sequencing in biomarker discovery in cancer research.

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Get started with NGS solutions for cancer research

Would your cancer research efforts benefit from sequencing? Are you just getting started in cancer research? See how you can easily improve your workflows and results.

xGen™ Exome Hyb Panel

The xGen Exome Research Panel v2 consists of 415,115 individually synthesized and quality controlled oligonucleotide probes. The xGen Exome Research Panel v2 spans a 34 Mb target region (19,433 genes) of the human genome and covers 39 Mb of end-to-end tiled probe space.

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xGen AML Cancer Hyb Panel

Predesigned panel for NGS research targeting >260 genes associated with AML for efficient identification of disease-causing mutations.

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xGen Custom Hyb Panels

xGen Custom Hyb Panels include your choice of high-fidelity, individually synthesized, 5’-biotinylated oligos for targeted NGS research. IDT proprietary DNA synthesis produces rapid, high-quality panels that can be optimized, expanded, and combined with other panels in research studies.

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xGen Inherited Diseases Hyb Panel

Predesigned panel with targeted enrichment of genes and single nucleotide polymorphisms (SNPs) associated with inherited diseases.

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EXPLORE NGS USES IN CANCER RESEARCH